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technology

NK Vue®

The Principle of NK VUE Diagnosis

NK VUE artificially activates the NK cells in the blood using our patented NK Cell activator (Promoca) and measures the amount of Interferon gamma secreted using the method of enzyme-linked immunosorbent assay (ELISA).

The Principle of NK VUE Diagnosis
  • Step1. Draw blood (1ml)
  • Step2. NK cells incubation (37°C, 20~24 hours)
  • Step3. IFN-γ secretion
  • Step4. Supernatant collected.
  • Step5. ELISA tested
  • Step6. Results verified

What is NK cell activity test?

This test measures the amount of Interferon gamma (IFN-γ) secreted after artificially activating NK cells in the blood.
It can help evaluate your body’s natural immunity against cancer or other diseases.

Difference in NK Cell Activity
Difference in NK Cell Activity between Cancer Patients and Healthy People
There is a significant difference in NK cell activity between cancer patients and healthy people.
Difference in NK Cell Activity by age or sex
There is no significant difference in NK cell activity depending on age or sex.

Clinical trial: 2011-2012, Yonsei University Severance Hospital Clinical Test Center

Comparison of NK VUE with traditional methods

NK VUE is much simpler to use compared to the traditional NK cell activity tests.

Traditional methods NK Vue methods
Flow cytometric Assay Chromium-51 Release Assay Flow cytometric Assay
(CD107a degranulation assay)
NK Vue®
Object NK Cell Number Counting NK Cell Cytotoxicity NK Cell Cytotoxicity as % NK Cell Activity as a number
Marker CD3, CD16/56 Chromium-51 CD3, CD16/CF56, CD107a IFN-γ
Blood Collection 2ml Variable Depends on NK Cell Number Variable Depends on NK Cell Number 1ml
Disadvantage
  • Only Cell Number counting
  • Radioactive isotopes PBMC Disadvantage
  • Lower reproducibility
  • Uses K562 cell
  • -Less sensitivity due to the lower number of analyte
  • Lower reproducibility
  • Uses K562 Cell
-
Sample Type Purified PBMC Purified PBMC Cultured lymphocytes Purified PBMC Whole Blood
Period 5 hours 5-7 days 10 hours 24-28 hours
(Including of 20-24 hours Incubating)
Accuracy Depends on Researcher Depends on Researcher Depends on Researcher Digitize through ELISA

Potential Application of NK Cell Activity Test in Various Areas

  • Pre-
    screening

    • Screening prior to PET-CT and CT examination
    • For biopsy pre-examination
    • Early detection of recurrence
    • Colorectal cancer pre-screening
      • Gastroenterology Journal in June 2017 (Canada)
      • JGH Journal in October 2017 (Korea)
  • Immunity
    Benchmark

    • Complement general health test such as DNA test, Antioxidant test, Biometric Age test
    • Regular health check-up
    • Hospital Health Center and Korea Medical Institutions
    • Korean Industrial Health Association and Korea Association of Health Promotions
  • Complementary
    Diagnosis

    • Tested with tumor marker tests
    • Sensitivity and specificity improvement
    • Prostate cancer
      • Can J Urol Journal in April 2017 (Canada)
    • Gastric cancer
      • Oncotarget Journal July 2017 (Korea)
  • Treatment
    Monitoring

    • Predicting effects on various drugs and treatments
    • Monitoring after chemotherapy
    • Monitoring Pre-chemotherapy treatment
      • ESMO Poster (European Society for Medical Oncology) in September 2017 (Spain)
      • ASCO Poster in June 2018 (U.S.A)

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