NK Vue®

The Principle of NK VUE Diagnosis

NK VUE artificially activates the NK cells in the blood using our patented NK Cell activator (Promoca) and measures the amount of Interferon gamma secreted using the method of enzyme-linked immunosorbent assay (ELISA).

What is NK cell activity test?

This test measures the amount of Interferon gamma (IFN-γ) secreted after artificially activating NK cells in the blood.
It can help evaluate your body’s natural immunity against cancer or other diseases.

Difference in NK Cell Activity between Cancer Patients and Healthy People

There is a significant difference in NK cell activity between cancer patients and healthy people.

Difference in NK Cell Activity by age or sex

There is no significant difference in NK cell activity depending on age or sex.

Clinical trial: 2011-2012, Yonsei University Severance Hospital Clinical Test Center

Comparison of NK VUE with traditional methods

NK VUE is much simpler to use compared to the traditional NK cell activity tests.

	Traditional methods			NK Vue methods
	Flow cytometric Assay	Chromium-51 Release Assay	Flow cytometric Assay (CD107a degranulation assay)	NK Vue®
Object	NK Cell Number Counting	NK Cell Cytotoxicity	NK Cell Cytotoxicity as %	NK Cell Activity as a number
Marker	CD3, CD16/56	Chromium-51	CD3, CD16/CF56, CD107a	IFN-γ
Blood Collection	2ml	Variable Depends on NK Cell Number	Variable Depends on NK Cell Number	1ml
Disadvantage	Only Cell Number counting	Radioactive isotopes PBMC Disadvantage Lower reproducibility Uses K562 cell	-Less sensitivity due to the lower number of analyte Lower reproducibility Uses K562 Cell	-
Sample Type	Purified PBMC	Purified PBMC Cultured lymphocytes	Purified PBMC	Whole Blood
Period	5 hours	5-7 days	10 hours	24-28 hours (Including of 20-24 hours Incubating)
Accuracy	Depends on Researcher	Depends on Researcher	Depends on Researcher	Digitize through ELISA

Potential Application of NK Cell Activity Test in Various Areas

Pre-
screening
- Screening prior to PET-CT and CT examination
- For biopsy pre-examination
- Early detection of recurrence
- Colorectal cancer pre-screening
  - Gastroenterology Journal in June 2017 (Canada)
  - JGH Journal in October 2017 (Korea)
Immunity
Benchmark
- Complement general health test such as DNA test, Antioxidant test, Biometric Age test
- Regular health check-up
- Hospital Health Center and Korea Medical Institutions
- Korean Industrial Health Association and Korea Association of Health Promotions
Complementary
Diagnosis
- Tested with tumor marker tests
- Sensitivity and specificity improvement
- Prostate cancer
  - Can J Urol Journal in April 2017 (Canada)
- Gastric cancer
  - Oncotarget Journal July 2017 (Korea)
Treatment
Monitoring
- Predicting effects on various drugs and treatments
- Monitoring after chemotherapy
- Monitoring Pre-chemotherapy treatment
  - ESMO Poster (European Society for Medical Oncology) in September 2017 (Spain)
  - ASCO Poster in June 2018 (U.S.A)

The following conditions or drugs have been known to temporarily affect immune cell function and significantly influence the test results:
Viral or bacterial infection / Autoimmune diseases. / Immunosuppressive drugs. / Acute Stress or Severe fatigue Sleep Disorders / Steroid-based drugs