NK VUE artificially activates the NK cells in the blood using our patented NK Cell activator (Promoca) and measures the amount of Interferon gamma secreted using the method of enzyme-linked immunosorbent assay (ELISA).
This test measures the amount of Interferon gamma (IFN-γ) secreted after artificially activating NK cells in the blood.
It can help evaluate your body’s natural immunity against cancer or other diseases.
Clinical trial: 2011-2012, Yonsei University Severance Hospital Clinical Test Center
NK VUE is much simpler to use compared to the conventional NK cell activity tests.
Conventional methods | NK Vue® method | |||
---|---|---|---|---|
Flow cytometric Assay | Chromium-51 Release Assay | Flow cytometric Assay (CD107a degranulation assay) |
NK Vue® Kit | |
Object | NK cell number counting | NK cell cytotoxicity | NK cell cytotoxicity | NK cell activity |
Marker | CD3, CD16/56 | 51Cr (radioisotope) | CD3, CD16/56, CD107a | IFN-γ |
Blood collection | 2mL | Variable depends on NK cell number | Variable depends on NK cell number | 1mL |
Sample Type | PBMC | PBMC or lymphocytes | PBMC | Whole blood |
Disadvantage | Only the relative percentage (%) of NK cells can be measured |
-Using radioisotope -Complicated experiments & low reproducibility -Expensive and time consuming |
Sensitivity and reproducibility may vary depending on the number of cells | - |
Pre-
screening
Immunity
Benchmark
Complementary
Diagnosis
Treatment
Monitoring
The following conditions or drugs have been known to temporarily affect immune cell function and significantly influence the test results:
Viral or bacterial infection / Autoimmune diseases. / Immunosuppressive drugs. / Acute Stress or Severe fatigue Sleep Disorders / Steroid-based drugs